Biomolecules are first converted into ions, and introduced into a mass spectrometer.
The ions of interest are then cooled to cryogenic temperatures using a compact cryocooler. Cooling significantly reduces thermal broadening, leading to high-resolution spectra.
Once cooled, the ions are fragmented by infrared or ultraviolet laser pulses at variable wavelength.
At each wavelength the fragment ions are monitored by high resolution MS. The recorded 2D UV/IR-MS fingerprint uniquely reflects structure of the ions.
Our 2D UV-MS fingerprinting method enables library-based and library-free identification and quantification of isomers in seconds
Cryogenic ion spectroscopy as an orthogonal dimension for metabolite analysis
Combining cryogenic infrared ion spectroscopy with quantum-chemical calculation allows for accurate elucidation of 3D molecular structure
This technique provides a unique look at the structure of biomolecules, helping scientists understand their functions and interactions
Cryogenic ion spectroscopy is highly versatile technology, applicable for identification and quantification of wide range of biomolecules and their isomers
Library-based identification and quantification can be done in seconds
Our technology works at MS level of sensitivity what is crucial for many life science applications
Cryogenic ion spectroscopy is compatible with LC-MS workflow
